Manipulating Embryos
Remove Jelly Coat from Embryos
How to remove the thick sticky protective coating that covers embryos. Embryos are difficult to manipulate and inject with the jelly coat intact.
Tables of Embryo Development
Tables of X. tropicalis and X. laevis development at different temperatures
Microinjection
Complete Protocol for microinjection of X. tropicalis embryos
Microinjection Protocol X. tropicalis
Microinjection differences between X. laevis and X. tropicalis
Protocol for creating transgenics with the Meganuclease enzyme
X. tropicalis Meganuclease Microinjection Protocol
Protocol to prepare and inject morpholino oligos to transiently knock down gene expression
Prepare RNA for overexpression studies
Sectioning Embryos
Sectioning allows better penetration of reagents and improves visualization of deeper tissues